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Human CD45 Protein

L-CA; CD45; T200; PTPRC; B220; CD45R; GP180; LCA; LY5; EC 3.1.3.48

Catalog No. Product Name Size List Price (US$) Quantity
BP004944 Human CD45/PTPRC Protein with the C-His Tag 100 ug 315.00
BP004944 Human CD45/PTPRC Protein with the C-His Tag 500 ug 1050.00
BP003518 Human CD45/PTPRC Protein with the C-hFc Tag 100 ug 315.00
BP003518 Human CD45/PTPRC Protein with the C-hFc Tag 500 ug 1050.00
BP004049 Human CD45/PTPRC Protein with the C-His-Avi Tag 100 ug 315.00
BP004049 Human CD45/PTPRC Protein with the C-His-Avi Tag 500 ug 1050.00
Description

BP004944: Human CD45/PTPRC Protein with the C-His Tag

Source: The recombinant Human CD45/PTPRC Protein, (amino acids Gln26-Lys577) fused with a His tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight: The recombinant Human CD45/PTPRC protein with the His tag at the C-terminus has a predicted MW of 61.9 kDa. Due to glycosylation, the protein migrates to 118-150 kDa based on Tris-Bis PAGE result.
Activity: Immobilized recombinant Human CD45 protein with the C-His tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Anti-CD45 Antibody protein with the C-hFc tag with the EC50 of 0.23 ug/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Human CD45/PTPRC Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.

BP003518: Human CD45/PTPRC Protein with the C-hFc Tag

Source: The recombinant Human CD45/PTPRC Protein, (amino acids Gln26-Lys577) fused with a hFc tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight: The recombinant Human CD45/PTPRC protein with the hFc tag at the C-terminus has a predicted MW of 87.53 kDa. Due to glycosylation, the protein migrates to 120-160 kDa based on Tris-Bis PAGE result.
Activity: Immobilized recombinant AHuman CD45 protein with the C-hFc tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Biotinylated Anti-CD45 Antibody protein with the C-hFc tag with the EC50 of 0.16 ug/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Human CD45/PTPRC Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.

BP004049: Human CD45/PTPRC Protein with the C-His-Avi Tag

Source: The recombinant Human CD45/PTPRC Protein, (amino acids Gln26­-Ile416) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight: The recombinant Human CD45/PTPRC protein with the His-Avi tag at the C-terminus has a predicted MW of 47.3 kDa. Due to glycosylation, the protein migrates to 80-110 kDa based on Tris-Bis PAGE result.
Activity: Immobilized recombinant Human CD45 protein with the C-His-Avi tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Anti-CD45 Antibody protein with the C-hFc tag with the EC50 of 16.9 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Human CD45/PTPRC Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.


Shipping: The recombinant Human CD45/PTPRC Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 week, 2 to 8°C under sterile conditions after reconstitution.
6 months, -20 to -70°C under sterile conditions after reconstitution.

Syd Labs provides the following CD45 Protein :
Biotinylated Human CD45 Protein
CD45 Protein
Human CD45 Protein
Mouse CD45 Protein

Syd Labs provides the following CD45 Antibody :
CD45 Antibody
PTPRC/CD45 Antibody

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